Born and raised in the outskirts of Athen, Despina and her sisters were raised to be good, civilized girls. Despina dreamt about the adventures of seaferring however, disobeying her father's wishes. Her mother only wanted the best for her daughter, and therefore found a well-educated man for Despina: Felix. At the age of 19, Despina were married and moved to the capital of Greece.
At the age of 24, Despina learned about her husband's allegiance to a secret society. Felix was a member of a group of men and women who worked under the name of Templars, and Despina were initiated to it after discussing what she could do for the Order. With his background, he established himself a trading-company. In the name of the Templar Order, Despina and her husband began earning a reputation as extreme people – doing nothing to stop them. While her husband stayed on the mainland, carrying out transactions for the Order and their puppets and allies, Despina raided non-greek trading ships – usually from the French or Spanish Empire – for their values, and brought them back to Greece. The greek Grand Master did not like her methods, but Despina defended herself by stating she did it in order to retrieve "certain artifacts". When she also managed to bring down a ship of the Greek Assassin Fleet – established with the help of a Welsh Master Assassin – Despina obtained three blood vials that the Templar scientist identified as those being used in the Observatory. After this incident, Despina were given permission to do as she pleased – as long as she did not attack other Templar-ships, or those belonging to Greece' allies.
Now operating under the cryptonym The Pirate, Despina was soon brought to the attention of the Murdress of Cyprus: Hacer Stavros. Operating contact with her, Despina began to act more and more on her behalf. Slowly, Despina became one of her lieutenants. Stavros' task was simple: sinking Assassin-ships and enemies of Cyprus. Despina's husband, Felix, soon moved to the city of Paphos, where he established a head quarter. Using five slums in Paphos as his eyes and ears, he earned the nickname The Crow – referring to a crow's nest on a ship.
After the fall of Stavros in 1734, the Greek Assassins Maria Nomiki and Sofia Sanna established an Assassin guild in the city of Kyrenia. Soon afterwards, the Greek Assassin Nikoleta Megalos were sent to kill Despina. Learning about the Templars' connections in Paphos however, Nikoleta firstly had to bring down each of the men and women The Crow had hired to supervise the slums. During one of her raids of the Cypriote Dens, Nikoleta met with the Israelite Rabi El-Ghazzawy – a man who later became Nikoleta's right-hand-man. After killing The Crow, Despina was the next target.
A few weeks after her husband's death, Despina – still unknowingly of this – landed in Paphos. On one of her encounters with the Frenchmen, she had obtained an artifact left behind of the First Civilization. It was nothing more but a manuscript that told about the Isu, but she found it interesting – wanting to decode it. Being escorted to Felix's head quarters, she was surprised to see it was overrun with Assassins. Not being able to flee, she were soon killed by an air assassination.
By killing Despina, the Cypriote Templars' lost their control of the smuggling and pirating in the Mediterranean. Because of this, pirates could be hired by Assassins and attack ships with Templar-cargo, or Templar-ships with valuable cargo. In order to not make the Templars regain power in the Paphos, Nikoleta stayed there with Rabi.